Mishanina, Tatiana
Transcription and its regulation; RNA polymerase structure and function in bacteria and human mitochondria
Transcription and its regulation; RNA polymerase structure and function in bacteria and human mitochondria
Contact Information
Assistant Professor of Chemistry and Biochemistry
Office: Natural Sciences Bldg 4320
Phone: 858-246-5883
Email: tmishanina@ucsd.edu
Web: mishaninalab.ucsd.edu
Group: View group members
Office: Natural Sciences Bldg 4320
Phone: 858-246-5883
Email: tmishanina@ucsd.edu
Web: mishaninalab.ucsd.edu
Group: View group members
Education
2014 Ph.D., Chemistry, University of Iowa
2008 B.S., Chemistry, Wichita State University
2014 Ph.D., Chemistry, University of Iowa
2008 B.S., Chemistry, Wichita State University
Appointments
2019–present Assistant Professor, Chemistry and Biochemistry, University of California San Diego
2015–2018 Postdoctoral Fellow, Biochemistry, University of Wisconsin–Madison
2014–2015 Postdoctoral Associate, Biological Chemistry, University of Michigan
2019–present Assistant Professor, Chemistry and Biochemistry, University of California San Diego
2015–2018 Postdoctoral Fellow, Biochemistry, University of Wisconsin–Madison
2014–2015 Postdoctoral Associate, Biological Chemistry, University of Michigan
Research Interests
Our lab is interested in the underlying molecular mechanisms of gene expression in human mitochondria and bacteria. We use a combination of biochemical and structural biology approaches to study how RNA polymerase, the central enzyme in gene expression, contributes to gene regulation via transcriptional pausing; how it couples RNA synthesis to DNA replication, RNA processing and translation in mitochondria; and how we can control these processes to rationally manipulate gene expression for antimicrobials and treatments of disease. Students in the lab are trained in enzymology (e.g., kinetics, in vitro assays) and state-of-the-art structural methods (e.g., single-particle cryo-EM).
Primary Research Area
Biochemistry
Biochemistry
Interdisciplinary interests
Macromolecular Structure
Cellular Biochemistry
Biophysics
Cellular Biochemistry
Biophysics
Selected Publications
- Kang JY,Mishanina TV,Landick R,Darst SA "Mechanisms of Transcriptional Pausing in Bacteria.", J Mol Biol, 2019, [View]
- Karunaratne K,Mishanina TV "Chemical quenching and identification of intermediates in flavoenzyme-catalyzed reactions.", Methods Enzymol, 2019, Vol. 620, 89-114 [View]
- Saba J,Chua XY,Mishanina TV,Nayak D,Windgassen TA,Mooney RA,Landick R "The elemental mechanism of transcriptional pausing.", Elife, 2019, Vol. 8, [View]
- Kang JY,Mishanina TV,Bellecourt MJ,Mooney RA,Darst SA,Landick R "RNA Polymerase Accommodates a Pause RNA Hairpin by Global Conformational Rearrangements that Prolong Pausing.", Mol Cell, 2018, Vol. 69, Issue 5, 802-815.e1 [View]
- Kang JY,Mooney RA,Nedialkov Y,Saba J,Mishanina TV,Artsimovitch I,Landick R,Darst SA "Structural Basis for Transcript Elongation Control by NusG Family Universal Regulators.", Cell, 2018, Vol. 173, Issue 7, 1650-1662.e14 [View]
- Mishanina TV,Palo MZ,Nayak D,Mooney RA,Landick R "Trigger loop of RNA polymerase is a positional, not acid-base, catalyst for both transcription and proofreading.", Proc Natl Acad Sci U S A, 2017, Vol. 114, Issue 26, E5103-E5112 [View]
- Mishanina TV,Yu L,Karunaratne K,Mondal D,Corcoran JM,Choi MA,Kohen A "An unprecedented mechanism of nucleotide methylation in organisms containing thyX.", Science, 2016, Vol. 351, Issue 6272, 507-10 [View]
- Mishanina TV,Kohen A "Synthesis and application of isotopically labeled flavin nucleotides.", J Labelled Comp Radiopharm, 2015, Vol. 58, Issue 9, 370-5 [View]
- Mishanina TV,Libiad M,Banerjee R "Biogenesis of reactive sulfur species for signaling by hydrogen sulfide oxidation pathways.", Nat Chem Biol, 2015, Vol. 11, Issue 7, 457-64 [View]
- Mishanina TV,Yadav PK,Ballou DP,Banerjee R "Transient Kinetic Analysis of Hydrogen Sulfide Oxidation Catalyzed by Human Sulfide Quinone Oxidoreductase.", J Biol Chem, 2015, Vol. 290, Issue 41, 25072-80 [View]
- Mishanina TV,Corcoran JM,Kohen A "Substrate activation in flavin-dependent thymidylate synthase.", J Am Chem Soc, 2014, Vol. 136, Issue 30, 10597-600 [View]
- Mishanina TV,Koehn EM,Conrad JA,Palfey BA,Lesley SA,Kohen A "Trapping of an intermediate in the reaction catalyzed by flavin-dependent thymidylate synthase.", J Am Chem Soc, 2012, Vol. 134, Issue 9, 4442-8 [View]
- Mishanina TV,Koehn EM,Kohen A "Mechanisms and inhibition of uracil methylating enzymes.", Bioorg Chem, 2012, Vol. 43, 37-43 [View]
- Cady SD,Mishanina TV,Hong M "Structure of amantadine-bound M2 transmembrane peptide of influenza A in lipid bilayers from magic-angle-spinning solid-state NMR: the role of Ser31 in amantadine binding.", J Mol Biol, 2009, Vol. 385, Issue 4, 1127-41 [View]
- Hong M,Mishanina TV,Cady SD "Accurate measurement of methyl 13C chemical shifts by solid-state NMR for the determination of protein side chain conformation: the influenza a M2 transmembrane peptide as an example.", J Am Chem Soc, 2009, Vol. 131, Issue 22, 7806-16 [View]